In vitro reconstruction of human junctional and sulcular epithelium
نویسندگان
چکیده
منابع مشابه
In vitro reconstruction of human junctional and sulcular epithelium
BACKGROUND The aim of this study was to develop and characterize standardized in vitro three-dimensional organotypic models of human junctional epithelium (JE) and sulcular epithelium (SE). METHODS Organotypic models were constructed by growing human normal gingival keratinocytes on top of collagen matrices populated with gingival fibroblasts (GF) or periodontal ligament fibroblasts (PLF). Ti...
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BACKGROUND This study aims to observe the morphological characteristics and identify the function characteristics of junctional epithelium (JE) tissues and cultured JE cells. METHODS Paraffin sections of human molar or premolar on the gingival buccolingual side were prepared from 6 subjects. HE staining and image analysis were performed to measure and compare the morphological difference amon...
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Matrilysin is a matrix metalloproteinase expressed in exocrine and mucosal epithelium in many human tissues. Immunohistochemical staining showed that matrilysin is expressed in suprabasal cells of junctional epithelium facing the teeth and in epithelial cell rests of Malassez. No matrilysin expression was seen in the periodontal pocket tissue. In a tissue culture model mimicking junctional epit...
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Expression, localization, and function of junctional adhesion molecule-C (JAM-C) in human retinal pigment epithelium.
PURPOSE To determine the localization of JAM-C in human RPE and characterize its functions. METHODS Immunofluorescence, Western blot, and PCR was used to identify the localization and expression of JAM-C, ZO-1, N-cadherin, and ezrin in cultures of human fetal RPE (hfRPE) with or without si-RNA mediated JAM-C knockdown and in adult native RPE wholemounts. A transepithelial migration assay was ...
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ژورنال
عنوان ژورنال: Journal of Oral Pathology & Medicine
سال: 2012
ISSN: 0904-2512,1600-0714
DOI: 10.1111/jop.12005